Figure 8.

Response of PDK4 and PDH to daily torpor in different tissues of gray mouse lemurs

The relative protein expression of PDH and phosphorylation of PDH at Ser232, Ser293, and Ser300) were assessed using bead-based assays and represented as relative MFI by comparing control (aroused) and torpor states. In addition, the expression of PDK4 was examined with ELISA using optical density as readout. The tissues examined include skeletal muscle (A), heart (B) liver (C), kidney (D), BAT (E), and WAT (F). Data were analyzed similarly as indicated in Figure 2. * Denotes significant difference from the corresponding control according to the two-tailed Student’s t-test (P < 0.05). MFI, median fluorescent intensity; BAT, brown adipose tissue; WAT, white adipose tissue; PDH, pyruvate dehydrogenase; PDK, pyruvate dehydrogenase kinase.