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. 2015 Jun 18;13(2):81–90. doi: 10.1016/j.gpb.2015.03.002

Figure 1.

Figure 1

Expression and phosphorylation of MAPK kinases and targets in skeletal muscle of gray mouse lemurs

A. Relative total protein levels of ERK, MEK, JNK, p38, HSP27, and p53. B. Relative ratio of phosphorylation levels (relative phosphorylation/relative total protein expression) of p-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187), p-MEK (Ser217/221), p-JNK (Thr183/Tyr185), p-p38 (Thr180/Tyr182), p-HSP27 (Ser78), and p-p53 (Ser15). All data were obtained by multiplex analysis using a Luminex 100 instrument and analyzed with Milliplex analyst software. Shown are histograms of mean of median fluorescent intensity (MFI) of immune-reactive multiplex beads (mean ± SEM), n = 4 independent samples from different animals from control (aroused) and torpid experimental conditions. Significant differences in torpid animals in comparison to controls are denoted with *(P < 0.05) and **(P < 0.01), respectively (two-tailed Student t-test).