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. 2015 Jul 23;9:36. doi: 10.3389/fncir.2015.00036

Figure 2.

Figure 2

Spinal and sensory neurons contribute to the DCN population. (A) For spinal DCN neurotracing, DiI crystals were placed in the dorsolateral region of the spinal cord. (B,C) At E17.5, DiI-labeled DCN axons cross in discrete bundles [arrowhead in (B)] and project longitudinally in both the rostral and caudal directions [arrowheads in (C)]. (D,E) In transverse sections, both DCN [white arrowhead in (D)] and commissural axons at the ventral midline (orange arrowhead) are labeled. spDCN cell bodies are labeled in the contralateral dorsolateral region of the spinal cord [white arrowheads in (E)]. (E) corresponds to boxed region in (D). (F) For snDCN neurotracing, DiI crystals were placed in DRG. (G,H) At P28, snDCNs cross the dorsal midline in two bundles, a posterior bundle (green arrowhead) and centrally at the dorsal midline (white arrowhead). snDCNs terminate within the dorsomedial and dorsolateral regions of the contralateral spinal cord. Sections from caudal thoracic and rostral lumbar spinal cord. (I) Summary of DCN populations. Predicted gray population indicates that DCNs are heterogeneous (see “Discussion” Section). Scale bars: 100 μm (B–D,G,H); 25 μm (E).