Table 1.
Comparison of Th1 and Th17 responses to whole MBP, MBP peptides and OVA in animals immunized with OVA or rat MBP and in animals subjected to MCAO. Data are displayed as median (interquartile range). Statistics are by Mann-Whitney U test.
| spleen | lymph node | brain | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Th1 | OV A N=14 |
MBP N=22 |
MCA O N=37 |
OV A N=10 |
MBP N=19 |
MCA O N=36 |
OV A N=11 |
MBP N=23 |
MCAO (ischemic hemisphere ) N=22 |
MCAO (non- ischemic hemisphere ) N=22 |
| rMBP | 0.80 (0.66, 1.06) | 0.98 (0.72, 1.34) | 0.82 (0.46, 1.75) | 0.67 (0.40, 1.05) | 1.35* (0.78, 2.00) | 1.26 (0.61, 2.13) | 0.74 (0.40, 1.04) | 1.26** (0.88, 1.43) | 0.88 (0.49, 1.68) | 0.89 (0.63, 1.51) |
| MBP 68-86 | 0.72 (0.54, 1.18) | 1.00 (0.55, 1.36) | 0.91 (0.48, 1.73) | 1.00 (0.55, 1.34) | 1.37 (0.80, 2.27) | 1.06 (0.56, 1.80) | 1.00 (0.66, 1.90) | 0.95 (0.86, 1.33) | 1.10 (0.56, 1.78) | 1.34 (0.71, 1.97) |
| MBP 63-81 | 0.70 (0.61, 1.36) | 1.01 (0.53, 1.45) | 0.84 (0.54, 1.86) | 0.75 (0.64, 1.83) | 1.00 (0.82, 1.34) | 1.05 (0.49, 1.86) | 0.91 (0.57, 1.08) | 1.16 (0.81, 1.45) | 0.91 (0.48, 1.22) | 1.41 (0.59, 2.35) |
| bMBP | 0.77 (0.64, 1.15) | 1.00 (0.66, 1.27) | 0.92 (0.56, 1.64) | 0.98 (0.70, 1.08) | 1.34 (0.77, 2.53) | 1.02 (0.61, 1.87) | 0.73 (0.31, 1.00) | 1.16** (0.92, 1.54) | 0.93 (0.55, 1.48) | 1.27 (0.57, 1.84) |
| hMBP | 1.02 (0.72, 1.24) | 0.88 (0.43, 1.97) | 1.02 (0.60, 1.58) | 0.75 (0.50, 1.34) | 2.15** (1.36, 3.33) | 0.89† (0.52, 2.02) | 0.64 (0.33, 1.17) | 1.44* (0.74, 2.68) | 0.95† (0.66, 1.33) | 1.18 (0.53, 2.32) |
| OVA | 0.88 (0.72, 1.27) | 1.03 (0.64, 1.64) | 0.93 (0.49, 1.58) | 1.00 (0.75, 2.91) | 1.35 (0.86, 3.79) | 0.98 (0.48, 1.49) | 0.62 (0.26, 0.92) | 0.92 (0.72, 1.96) | 0.90 (0.39, 1.56) | 0.97* (0.67, 1.54) |
| Th17 |
OVA N=14 |
MBP N=22 |
MCAO N=37 |
OVA N=10 |
MBP N=19 |
MCAO N=36 |
OVA N=11 |
MBP N=23 |
MCAO (ischemic hemisphere) N=22 |
MCAO (non-ischemic hemisphere) N=22 |
| rMBP | 0.60 (0.44, 0.76) | 0.84** (0.70, 1.18) | 0.85* (0.45, 1.47) | 0.89 (0.62, 1.00) | 1.69** (1.00, 2.39) | 0.76† (0.40, 2.00) | 0.79 (0.56, 0.99) | 1.47** (1.20, 1.96) | 1.11† (0.41, 1.78) | 0.80‡ (0.45, 1.46) |
| MBP 68-86 | 0.66 (0.27, 1.18) | 1.26* (0.76, 2.40) | 0.84 (0.53, 1.40) | 1.00 (0.70, 1.74) | 1.50 (0.89, 2.30) | 0.67† (0.34, 1.46) | 0.98 (0.58, 1.38) | 1.42 (0.88, 2.02) | 1.10 (0.40, 1.85) | 1.05 (0.54, 2.16) |
| MBP 63-81 | 0.61 (0.35, 1.26) | 0.98 (0.63, 2.10) | 0.88 (0.56, 1.24) | 1.00 (0.68, 1.94) | 0.95 (0.43, 1.69) | 0.89 (0.37, 1.61) | 1.04 (0.46, 1.55) | 1.67 (1.10, 2.15) | 0.82 (0.44, 1.86) | 0.97† (0.47, 1.37) |
| bMBP | 0.57 (0.30, 0.77) | 0.93* (0.62, 1.18) | 0.92* (0.56, 1.64) | 1.00 (0.34, 1.08) | 1.00 (0.71, 1.48) | 0.69 (0.44, 1.50) | 1.00 (0.65, 1.31) | 1.41* (1.23, 2.01) | 0.83 (0.34, 2.13) | 1.36 (0.62, 2.29) |
| hMBP | 0.72 (0.39, 0.85) | 0.85 (0.69, 1.48) | 0.92 (0.58, 1.55) | 0.94 (0.55, 1.00) | 1.35* (0.80, 2.89) | 0.95 (0.52, 1.80) | 1.00 (0.44, 1.31) | 1.74** (1.20, 3.83) | 0.83‡ (0.38, 2.19) | 1.03† (0.64, 2.05) |
| OVA | 0.80 (0.68, 0.98) | 1.01 (0.80, 1.35) | 0.71 (0.55, 1.16) | 1.00 (0.75, 1.74) | 1.31 (0.56, 1.59) | 0.87 (0.46, 1.52) | 0.94 (0.30, 1.90) | 1.24 (0.58, 1.90) | 1.13 (0.45, 2.06) | 1.24* (0.62, 2.43) |
Note: In a subset of animals undergoing MCAO (N=15), brains were removed and frozen. Lymphocytes are thus not available from these brains.
MBP=myelin basic protein, rMBP=rat MBP, bMBP=bovine MBP, hMBP=human MBP, ova=ovalbumin, MCAO=middle cerebral artery occlusion.
*
signifies P≤0.05 compared to OVA immunized animals;
**
signifies P≤0.001 compared to OVA immunized animals,
†
signifies P≤0.05 compared to rMBP immunized animals,
‡
signifies P≤0.001 compared to rMBP immunized animals.