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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Mar 16;13(5):913–922. doi: 10.1158/1541-7786.MCR-14-0680

Figure 2.

Figure 2

Chemiluminescence assay of PL-NKX3.1 degradation in 96-well plates. A. Schematic diagram of the siRNA strategy for kinome siRNA screening. From master plates, four target-specific siRNAs were pooled into working transfection plates, siRNA pools from each transfection plate were reverse transfected into LNCaP-PLNKX7 cells. After 72 h of transfection, the plates were treated with cycloheximide for 1 hr. Chemiluminescent assay was performed with Prolabel Detection Kit II. B. Plate-to-plate reproducibility is shown in a pairwise plot of Z-score values from replicate plates 7a and 7b that were the master plates used for first round screening. Cells were plated in a replicate manner, but the transfections were performed separately at different times. Linear regression indices of the pairwise comparison are shown.