Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Mar 16;13(5):913–922. doi: 10.1158/1541-7786.MCR-14-0680

Figure 3.

Figure 3

A. NKX3.1 expression levels in duplicate assays of the second round screening. Independent result are shown in blue and red, representing the mean of four individual siRNA transfections. For mock transfection (Ctrl), cells were treated with or without 100 μg/ml cycloheximide for 60 min. Gene symbols in red are those chosen for final validations. B. Expression of endogenous kinase expression in LNCaP cells. Except BLK (B lymphoid tyrosine kinase), the expression of all other kinase candidate mRNAs was easily detected with semi-quantitative RT-PCR. C. Custom siRNAs were used to knock down kinase expression in LNCaP cells. RT-PCR was performed. For semiquantitative RT-PCR reaction cycles were as follows: PRKAR1A: 25 cycles; PIK3R1: 26 cycles; DYRK1B: 28 cycles; MARK2 and WNK3: 30 cycles; MAP4K1: 31 cycles; CDKN2A: 32 cycles; GRK7: 34 cycles.