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Kinase knockdown of NKX3.1 expression. A. LNCaP cells were transfected with control or kinase siRNA. Cycloheximide 100 μg/ml was added at 72 h after and cell extracts were prepared at the indicated times for immunoblots. B. Immunoblots were quantitated with Image J and NKX3.1 half-life was determined using Graph Pad Prism.
