Figure 2. IRGM1 promotes B16 melanoma cell migration and invasion.

B16 cells were transfected with either control or IRGM1siRNA (a,c) or transduced with either GFP or IRGM1-GFP lentiviral vectors (b,d). (a,b) B16 cells (5 × 10³) were then seeded on top of the Boyden chambers. After 24 hrs, B16 cells on the bottom were stained with 1% crystal violet. (c,d) B16 cells (5 × 10³) were seeded into Boyden chambers coated with Matrigel. After 48 hrs, cells on the underside of the membranes were fixed and then stained with crystal violet. Y-axis represents stained cell count per field. Data represent three independent experiments. (Unpaired t-test; *p < 0.05, **p < 0.01, ***p < 0.001).