Table 1.
Comparison of phenotypic and molecular assays apllied to bloodculture bottles
| Phenotipyc detection and identification by phenotypic analisys (n = 124) | Molecucar detection by GP/GN multiplex PCR | Concordance (%) | Molecular identification by specific-species qPCR (n = 124) | Concordance (%) | Average Cycle threshold | |
|---|---|---|---|---|---|---|
| S. aureus | 12 | 12 | 100 % | 10 | 83.40 % | 17,683 |
| SCoN | 35 | 28 | 80 % | 32 | 91.40 % | 20,608 |
| Enterococcus spp. | 5 | 5 | 100 % | 5 | 100 % | 24,923 |
| S. pneumoniae | 1 | 1 | 100 % | 1 | 100 % | 20,977 |
| K. pneumoniae | 3 | 3 | 100 % | 3 | 100 % | 29,111 |
| E. cloacae | 6 | 6 | 100 % | 6 | 100 % | 20,919 |
| E. coli | 8 | 7 | 87.50 % | 7 | 87.50 % | 18,995 |
| S. marcescens | 2 | 2 | 100 % | 2 | 100 % | 25,252 |
| P. aeruginosa | 14 | 13 | 92.80 % | 13 | 92.80 % | 19,395 |
| S. malthofilia | 1 | 1 | 100 % | 1 | 100 % | 36,974 |
| Acinetobacter spp. | 1 | 1 | 100 % | 1 | 100 % | 27,993 |
| Candida spp. | 12 | 12 | 100 % | 3 | 25 % | 25,464 |
| Other fungi | 2 | 2 | 100 % | a | a | a |
| Streptococcus sp. | 9 | 7 | 77.80 % | a | a | a |
| Others | 13 | 12 | 92.30 % | a | a | a |
aSpecies not included in the in-house qPCR standardized assay described in this study