Table 1.
Target genes and primers used to identify adherent Escherichia coli obtained from children diarrhoea in the Brazilian Amazon.
| Target | Gene | Primer | EP | Reference |
|---|---|---|---|---|
| EPEC | eaeA |
fp : 5′-TGAGCGGCTGGCATGAGTCATAC-3′
bp : 5′-TCGATCCCCATCGTCACCAGAGG-3′ |
241 | Pass et al. (2000) |
| EPEC | bfp |
fp : 5′-AATGGTGCTTGCGCTTGCTGC-3′
bp : 5′-GCCGCTTTATCCAACCTGGTA-3′ |
324 | Gunzburg et al. (1995) |
| EAEC | eagg |
fp : 5′-AGACTCTGGCGAAAGACTGTATC-3′
bp : 5′-ATGGCTGTCTGTAATAGATGAGAAC-3′ |
194 | Pass et al. (2000) |
| EAEC | aggA |
fb : 5′-GCTAACGCTGCGTTAGAAAGACC-3′
bp : 5′-GGAGTATCATTCTATATTCGCC-3′ |
352 | Piva et al. (2003) |
| EAEC | aafA |
fp : 5′ - GACAACCGCAACGCTGCGCTG-3′
p : 5′-GATAGCCGGTGTAATTGAGCC-3′ |
307 | Piva et al. (2003) |
Note - EP: expected product; fp, forward primer; bp, backward primer.