Table 1.

Summary of the steps for phytase purification from Aspergillus niger UFV-1.

Purification step	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Purification (fold)	Yield (%)
Crude extract	47.70	138.6	2.9	1	100
Ultra-filtration	41.30	129.6	3.14	1.08	93.5
Acid treatment	15.36	103.7	6.75	2.33	74.8
DEAE-Sepharose CL-6B	0.988	24.50	24.80	8.55	17.7
Sephacryl S-300 HR	0.082	15.30	186.6	64.34	11.0