Table 3.

Substrate specificity of the purified phytase from Aspergillus niger UFV-1.

Substrate	Relative activity a (%)
Sodium phytate	100.0 ± 1.3
p -NPP	384.1 ± 2.3
2-naphthyl phosphate	377.2 ± 3.4
1-naphthyl phosphate	359.1 ± 3.3
ATP	343.6 ± 3.6
Phenyl phosphate	272.8 ± 2.5
Glucose 6-phosphate	189.2 ± 2.4
ADP	101.7 ± 1.7
Calcium phytate	97.0 ± 1.2
Glycerol 2-phosphate	67.3 ± 0.3
Glucose 1-phosphate	58.2 ± 2.8
AMP	11.0 ± 0.3

^aPhytase activity was expressed as the mean ± SD (n = 3).