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Partial purification of extracellular laccase from Aspergillus flavus grown in basal GYP medium amended with 1 mM copper sulfate.
| Fraction | Volume (mL) | Activity (U/mL) | Protein (μg/mL) | Specific activity (U/μg) | Purification fold |
|---|---|---|---|---|---|
| Culture filtrate | 1200 | 320.85 | 401 | 0.8 | 1 |
| Ultrafiltration | 100 | 157.025 | 18 | 8.72 | 11 |
| Gel filtration, Sephadex G-100 | |||||
| Fractions:L1 | 15 | 25.92 | 6 | 4.32 | 5.5 |
| L2 | 10 | 79.57 | 1.24 | 64.17 | 81 |
