Figure 2. Coomassie-stained SDS-PAGE and zymogram gel on 12% PAA-gels visualizing recombinant AprX and LipM. Lane S: molecular mass standard (Biorad); lane 1: SDS-PAGE of crude extract of E. coli XL1-Blue carrying pQE30-Xa-aprX-041; lane 2: SDS-PAGE of crude extract of E. coli XL1-Blue carrying pQE30-Xa-lipM-041; lane 3: SDS-PAGE of purified AprX; lane 4: azocasein zymogram of purified AprX; lane 5: SDS-PAGE of purified LipM; lane 6: MU-butyrate zymogram of purified LipM.
