| Strains |
|
E. coli
XL1-Blue
|
Cloning and subcloning host sup
E44, hsd
R17, end
A1, rec
A1, g
yr
A96, thi
1, rel
A1, lac
-F[
pro
AB+, lac
Iq, lac
Z-M15, Tn10 (
tet
F)]
|
Bullock et al., 1987
|
|
P. fluorescens
07A
|
Wild type |
Martins et al.
, 2005
|
|
P. fluorescens
041
|
Wild type |
Martins et al.
, 2005
|
| Plasmids |
| pCR2.1-TOPO |
Cloning vector, lacZ
α fragment containing MCS, f1 origin, ColE1, Km
r
Ap
r
|
Invitrogen |
| pQE30-Xa |
Vector for the insertion of a Factor Xa Protease recognition site C-terminal of the 6xHis tag, T5 promoter, lac
operator, ribosome binding site, ATG start codon, His tag sequence, multiple cloning sites, stop codons in all three reading frames, Col E1 origin of replication, Ap
r
|
Qiagen |
| pQE30-Xa-aprX041 |
1.43 kb fragment containing aprX
from P. fluorescens
041 in pQE30-Xa, Ap
r
|
This study |
| pQE30-Xa-lipM041 |
1.42 kb fragment containing lipM
from P. fluorescens
041 in pQE30-Xa, Ap
r
|
This study |
