Table 2. Primers used to amplify the aprX and lipM gene by PCR.

Primer	Sequence (5′-3′)	Aplication
Apr-F	TTATGTCAAAAGTAAAAGAC	Amplification of aprX gene
Apr-R	TCAGGCTACGATGTCACTG	Amplification of aprX gene
APRX-F	ATT GGATCC AAAGCTATTGTATCTGCCGCG	Amplification of aprX gene and preparation for cloning in pQE-30Xa
APRX-R	ATT GAGCTC TCAGGCTACGATGTCACTGGC	Amplification of aprX gene and preparation for cloning in pQE-30Xa
Lip-F	ATGGGTRTSTTYGACTATAAAAACC	Amplification of lipM gene
Lip-R	TTAACCGATCACAATCCCCTCC	Amplification of lipM gene
LIPM-F	ATT GGATCC AACCTCGGTACCGAGGACTC	Amplification of lipM gene and preparation for cloning in pQE-30Xa
LIPM-R	ATT GAGCTC TTAACCGATCACAATCCCCTCCC	Amplification of lipM gene and preparation for cloning in pQE-30Xa

The introduced restriction sites BamHI and Sac I are underlined.