. 2015 Mar 31;46(1):207–217. doi: 10.1590/S1517-838246120130859

Table 3. Effect of metal ions on the activities of alkaline metalloprotease and lipase.

Metal ion	Relative activity (%)

	Alkaline metalloprotease ^a	Lipase ^b
None	100 ± 2	100 ± 2
Mn ²⁺	73 ± 1	61 ± 1
Co ²⁺	48 ± 3	59 ± 8
Zn ²⁺	86 ± 3	49 ± 0
Fe ²⁺	90 ± 5	48 ± 3
Fe ³⁺	102 ± 1	65 ± 2
Mg ²⁺	100 ± 1	50 ± 3

A reaction mixture containing 250 μL of 2% (w/v) azocasein in 50 mM Tris/HCl (pH 8.0), 75 μL of AprX, and 1 mM of each metal ion was incubated at 37 °C for 12 h. The remaining activity was then measured, as described in the text. Results show the mean value (n = 3) plus or minus the standard deviation.

A reaction mixture containing 1 mL of substrate (one volume of 0.3% (w/v) p -nitrophenyl palmitate in isopropanol and nine volumes 0.2% (w/v) sodium desoxycholate and 0.1% (w/v) gummi arabicum in 50 mM sodium phosphate buffer, pH 8.0), 50 μL of LipM, and 1 mM of each metal ions was incubated at 25 °C for 20 min. The remaining activity was then measured, as described in the text. Results show the mean value (n = 3) plus or minus the standard deviation.