Table 3.
List of proteins whose levels were significantly altered in the kidneys as a function of time following treatment of mdx23 mouse with a single high dose PMO (800 mg/kg) or saline solution alone.
| Uniprot ID | Description | Day 2 |
Day 7 |
Day 30 |
Function | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Saline | PMO | Fold (PMO/saline) | Saline | PMO | Fold (PMO/saline) | Saline | PMO | Fold (PMO/saline) | |||
| Q8K010 | 5-OPase | 1.96 ± 0.09 | 4.96 ± 0.13 | 2.5 | 2 ± 0.12 | 4.98 ± 0.28 | 2.49 | 2.44 ± 0.05 | 2.02 ± 0.4 | 0.83 | Catalyzes 5-oxo-l-proline to form l-glutamate, ATP to ADP |
| P00405 | Cytcox | 2.32 ± 0.6 | 5.56 ± 0.53 | 2.4 | 2.43 ± 0.2 | 7.52 ± 0.88 | 3.09 | 2.01 ± 0.17 | 3.6 ± 0.53 | 0.56 | The component of the respiratory chain |
| Q05920 | PCB | 2.94 ± 0.83 | 6.09 ± 0.48 | 2.1 | 3.35 ± 0.05 | 9.65 ± 0.25 | 2.74 | 3.17 ± 0.19 | 2.29 ± 0.02 | 0.58 | ATP + pyruvate + HCO3− = ADP + phosphate + oxaloacetate |
| P05202 | mAspAT | 1.15 ± 0.06 | 2.31 ± 0.09 | 2 | 1.44 ± 0.07 | 4.3 ± 0.69 | 2.99 | 1.94 ± 0.15 | 1.1 ± 0.08 | 0.57 | Metabolite exchange between mitochondria and cytosol |
| Q99K67 | Aass | 1.54 ± 0.04 | 3.13 ± 0.3 | 2 | 1.63 ± 0.35 | 5.62 ± 0.48 | 3.45 | 2.9 ± 0.3 | 2.02 ± 0.09 | 0.70 | Bifunctional enzyme |
| P07356 | Anx2 | 1.37 ± 0.1 | 3.03 ± 0.3 | 2.21 | 1.24 ± 0 | 3.46 ± 0.11 | 2.79 | 3.83 ± 0.17 | 1.63 ± 0.12 | 0.44 | Calcium-regulated membrane-binding protein |
| P16858 | GAPDH | 1.29 ± 0.06 | 2.36 ± 0.19 | 1.83 | 1.59 ± 0.13 | 3.73 ± 0.27 | 2.35 | 1.72 ± 0.1 | 1.22 ± 0.05 | 0.71 | playing a role in glycolysis and nuclear functions |
| Q9CQJ8 | CI-B22 | 2.11 ± 0.17 | 1.25 ± 2.9 | 0.78 | 0.89 ± 0.08 | 10.23 ± 0 | 11.49 | 2.92 ± 0.94 | 2.29 ± 0.06 | 0.59 | Mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P11404 | FABPH | 2.22 ± 0 | 1.66 ± 0.02 | 1.08 | 0.44 ± 1.26 | 1.62 ± 0 | 3.68 | 1.93 ± 0.31 | 1.5 ± 0.09 | 0.74 | A role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters |
| P28825 | MEP-1a | 1.78 ± 0.2 | 2.12 ± 0.02 | 1.19 | 1.11 ± 0.98 | 3.55 ± 0.93 | 3.20 | 2.05 ± 0.23 | 1.53 ± 0.14 | 0.75 | Kidney proximal tubules intestinal brush borders and salivary ducts |
| P97449 | AP-Na | 2.09 ± 0.07 | 3.47 ± 0.08 | 1.66 | 2.03 ± 0.36 | 6.61 ± 0.85 | 3.26 | 2.41 ± 0.22 | 1.66 ± 0.01 | 0.68 | proximal convoluted tubules |
| P16406 | AP-Aa | 1.64 ± 0 | 3.07 ± 2.82 | 0.70 | 1.87 ± 0.22 | 6.51 ± 0.03 | 3.48 | 2.04 ± 0.03 | 1.43 ± 0.04 | 1.87 | A role in the catabolic pathway of the renin-angiotensin system |
Top row indicates the days after single injection of high dose PMO or saline solutions. Abbreviations: 5-OPase for 5-oxoprolinase; Cytcox for Cytochrome c oxidase subunit 2; PCB for mitochondrial pyruvate carboxylase; mAspAT for mitochondrial aspartate aminotransferase; GAPDH for glyceraldehyde-3-phosphate dehydrogenase; Aass for alpha-aminoadipic semialdehyde synthase; Anx2 for annexin 2; CI-B22 for NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; FABPH for fatty acid binding protein heart type; AP-N for aminopeptidase N; AP-A for glutamyl aminopeptidase; Mep-1 for Meprin A subunit alpha; PMO: phosphorodiamidate morpholino oligomer. Data are presented as mean ± standard deviation of n = 1 per group and per time point. The means ± SD represent the average of light to heavy isotope ratio values obtained for all peptides detected for each protein in each sample.
Known biomarkers associated with kidney injury.