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. 2015 May 13;64(8):2780–2790. doi: 10.2337/db14-1772

Figure 2.

Figure 2

Effect of HF diet on insulin and glucose tolerance and on CEACAM1 protein levels. A: BL6 mice (2 months old) were fed an RD (■) or an HF diet (□) for 12–28 days before they were challenged with an intraperitoneal injection of glucose (1.5 g/kg BW) (i) or insulin (0.75 units/kg body weight) (ii) to assess blood glucose levels at 0–180 min postinjection. *P < 0.05 HF vs. RD (n > 6 per feeding group). B: Liver lysates were subjected to Northern (i) and Western (ii) analyses and reprobing with β-actin cDNA and α-actin antibody, respectively, for normalization (n > 6 mice per feeding group). Bands were scanned, and the density was measured with arbitrary units (AU) and represented in the right-hand graphs. The numbers above the gel denote different mice. *P < 0.05 vs. RD. C: Kidney lysates from three mice per feeding group were subjected to sequential Ib with α-mouse CEACAM1 antibody, followed by reprobing (reIb) with α-actin antibody for normalization.