Increased TAG turnover during reperfusion in MHC-DGAT1 hearts. A: TAG content measured in lipid extracts from perfused hearts freeze-clamped at the end of baseline (BL), ischemia (ISC), or reperfusion (REP) (n = 3–8 per group at each time point). B: Total incorporation rate of LCFAs into the TAG pool during reperfusion (n = 6–7). C: Incorporation rates of the individual fatty acids into the TAG pool during reperfusion in isolated perfused hearts. Rates for palmitate (16:0), palmitoleate (16:1), stearate (18:0), oleate (18:1), linoleate (18:2), and linolenate (18:3) are shown (n = 6–7 for each). D: Percent oxidation of fatty acids (FA), glucose (GLC), and unlabeled substrates (UNL) (lactate, glycogen, and TAG) during reperfusion in extracts from isolated perfused hearts analyzed by 13C NMR spectroscopy (n = 6–7). *P < 0.05 vs. CON; #P < 0.05 vs. MHC-DGAT1 (DGAT) at BL.