Figure 6.

Palmitate impairs functional recovery in MHC-DGAT1 hearts. A: RPP measured at the end of baseline equilibration (BL) and at the end of reperfusion (REP) in isolated perfused hearts from CON and MHC-DGAT1 (DGAT) hearts. Hearts were perfused with a mixed substrate buffer consisting of 5.5 mmol/L glucose, 0.4 mmol/L palmitate, 1.2 mmol/L lactate, and 50 μU/mL insulin during the entire protocol (n = 7 each group). B: Percent oxidation of fatty acids (FA), glucose (GLC), and unlabeled substrates (UNL) (lactate, glycogen, and TAG) during reperfusion in extracts from isolated perfused hearts analyzed by ¹³C NMR spectroscopy (n = 3–4). C–E: PCr, ATP, and Pi content measured with ³¹P NMR spectroscopy in isolated perfused hearts at the end of baseline equilibration, end of ischemia (ISC), and end of reperfusion (n = 4 each group at each time point). F: Intracellular pH assessed by ³¹P NMR spectroscopy in isolated perfused hearts, calculated by the chemical shift between Pi and PCr (n = 4 each group). *P < 0.05 vs. CON.