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. Author manuscript; available in PMC: 2015 Jul 23.
Published in final edited form as: J Urol. 2015 Feb 11;194(1):230–237. doi: 10.1016/j.juro.2015.02.036

Figure 1. Effect of MMC and siRNA on survivin expression in vitro.

Figure 1

RT4 cells were incubated for 4 hr in transfection medium (control), with PPCat-siNT (100 nM) or PPCat-siSurvivin (100 nM), followed by 2 hr treatment with complete medium or MMC (0.1 and 3 µM). At 48 hr post-treatment, cells were collected and analyzed for survivin mRNA and protein levels. Ctl: control. –si: no siRNA, siNT: nontarget siRNA, siSur: survivin siRNA. Data for mRNA levels are mean+1 SD (n=3 experiments, with triplicate samples per experiment). Data for protein levels are mean+1 SD (n=3 experiments, single sample per experiment). *p<0.05 compared to control. **p<0.05 compared to MMC and MMC+PPCat-siNT.