Table 3. msh3Δ microcolony tract dynamics.
TNR tract dynamics of individual msh3Δ microcolonies | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Day | A | B | C | D | E | F | G | H | I | J | K | L | M | Na | O | P | Qb | R | S | T | U | V | W | |
Initial tractc | 30 | 31 | 30 | 30 | 31 | 32 | 32 | 32 | 32 | 29 | 30 | 30 | 32 | 30 | 30 | 36 | 29 | 29 | 30 | 30 | 29 | 29 | 30 | |
1 | 0d | 0 | 0 | 0 | 0 | −3 | −2 | −2 | 0 | 0 | 0 | 0 | −2 | −1 | 0 | — | 0 | 0 | 0 | 0 | 0 | −2 | 0 | |
2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | —e | — | — | — | — | — | — | — | — | — | −5 | 0 | −5 | −5 | — | 0 | 0 | 0 | — | — | 0 | — | — | |
4 | — | — | — | — | 0 | 0 | 0 | −1 | — | — | 0 | 0 | 0 | 5 | — | — | 0 | — | — | — | 0 | — | — | |
5 | — | — | — | — | — | — | — | — | — | — | — | — | — | 0 | — | — | 0 | — | — | 0 | 0 | — | — | |
6 | 0 | — | 0 | 0 | 0 | 0 | — | 0 | 0 | — | 5 | 0 | 5 | 0 | 0 | — | 0 | — | — | 0 | — | — | — | |
7 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | — | 0 | 0 | 0 | 0 | 0 | 0 | — | 0 | 0 | |
8 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | — | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | |
9 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | −3 | 0 | 0 | 0 | 0 | 1 | 0 | — | 0 | 0 | — | 0 | 0 | 0 | 0 | |
10 | 0 | 0 | 0 | −5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | — | −1 | |
11 | 0 | 0 | 0 | 1 | — | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | — | — | 0 | — | 0 | 0 | — | — | — | −4 | |
Final tractf | 29 | 31 | 30 | 30 | 31 | 29 | 30 | 31 | 32 | 29 | 30 | 30 | 30 | 29 | 30 | 29 | 29 | 29 | 30 | 30 | 29 | 31 | 28 | |
Net changeg | −1 | 0 | 0 | 0 | 0 | −3 | −2 | −1 | 0 | 0 | 0 | 0 | −2 | −1 | 0 | −7 | 0 | 0 | 0 | 0 | 0 | 0 | −2 |
Corresponds to gel in upper panel of Figure 11B.
Corresponds to gel in lower panel of Figure 11B.
Initial expansion size following selection in the presence of 5-FOA in number of repeats.
Change in number of repeats in tract.
No amplification of tract.
Number of repeats.
Net change in number of repeats within tract.