Figure 4.

CDK5 induces DRP1 recruitment from the cytosol to the mitochondria. (A) Cerebellar granule neurons were infected with adenoviruses carrying expression cassettes for either of GFP control or cytoplasmic CDK5 at 1 DIV. The protein lysates were collected at 3 DIV and were subjected to cell fractionation. Cytoplasmic and mitochondrial fractions were analyzed by immunoblotting using indicated antibodies. Complex V and GAPDH were used as control for mitochondrial and cytoplasmic fractions, respectively. (B) Quantification of the relative densitometric values of DRP1 and phospho-DRP1 is shown for neurons expressing GFP control or CDK5-NES. **P < 0.01.