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. 2015 May 20;24(16):4584–4598. doi: 10.1093/hmg/ddv189

Figure 3.

Figure 3.

R243L ADNIV mutation increases catalytic activity of a recombinant mini-CAPN1/5 hybrid. The R243L mutation renders the mini-CAPN1/5 hybrid over 300% more sensitive to calcium (activity is a ratio normalized to mini-CAPN1; control is the reaction without calpain). (A) A time-course comparison of the CAPN1/5 hybrid and a hybrid bearing the R243L mutation, showing catalysis of a standard peptide substrate at 0.01 mm calcium, (B) 0.10 mm calcium, (C) 1.0 mm calcium and (D) 100 mm calcium. RFU, relative fluorescent units.