Figure 4.
Transgenic ADNIV mutant construct. (A) A p4.2 construct designed for opsin promoter-driven expression of a Myc-tagged human CAPN5R243L transgene (pA, polyadenylation signal; AmpR, ampicillin-resistant selection gene; pUC ori, origin of replication). (B) The hCAPN5R243L transgene expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in two transgenic mice as compared with a WT control mouse and a non-transgenic mouse from the same litter. (C) Anti-Myc immunoblot confirms transgene expression in the retinal extract.