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. 2015 Jul 23;10(7):e0133472. doi: 10.1371/journal.pone.0133472

Fig 6. Tamoxifen has little effect on Myh6 MerCreMer/+ hearts.

Fig 6

Apoptosis and fibrosis assays on Myh6 MerCreMer/+ mouse hearts in one week (A-F) and five weeks (G-L) after tamoxifen injection (0.01 mg/g body weight for 3 days). A few apoptotic cells were observed on both R26R tdTomato/+ and Myh6 MerCreMer/+;R26R tdTomato/+ hearts after one week (arrows in A,B, P60→P67), and very few detected after five weeks (G,H, P60→P95). Tamoxifen mediates robust recombination on Myh6 MerCreMer/+;R26R tdTomato/+ hearts (D,J), but not on R26R tdTomato/+ control hearts (C,I). Myocardial fibrosis was not detected on R26R tdTomato/+ or Myh6 MerCreMer/+;R26R tdTomato/+ hearts by trichrome staining after one (E,F) and five weeks (K,L). Image in the upright corner of E is from an unrelated study, showing positive trichrome staining and cardiac fibrosis (arrows). This staining was performed in parallel with sample in E.