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. Author manuscript; available in PMC: 2015 Jul 23.
Published in final edited form as: Dig Dis Sci. 2014 May 3;59(6):1197–1206. doi: 10.1007/s10620-014-3167-6

Fig. 5. Markers of HSC activation and collagen deposition are markedly decreased in Casp3−/− mice compared to WT mice on MCD diet.

Fig. 5

Quantification of collagen fibers was performed in liver section stained with Sirius red (A) (magnification 40x) and quantified using the surface area stained per field area ± S.D. (n = 4–6 in each group) excluding blood vessels (B). Immunofluorescence staining of α-SMA was also performed on frozen liver section (C). α-SMA was detected using western blot assay from liver homogenates and densitometry analysis of α-SMA over α-tubulin was analyzed (D). Quantitative RT PCR analysis of HSC activation markers alpha-smooth muscle actin ( -SMA), collagen, type I-alpha (COL1A1), and transforming growth factor-beta (TGF- ) mRNA expression was assessed in WT and Casp3−/− mice on the CTL and MCD diet (n = 4–5 in each group). Results are expressed as mean ± SEM. *p-value < 0.05.