Table 2. Allele specific primers used for gene expression analysis.

Gene	Heterozygote position	Primer sequence (5′-3′)	TA (°C)	Product size (bp)
Ecdysone 20 monooxygenase like	48 (A/G)	F1: GCGGAAGCCGTCAGG	58	34
		F2: TTAGCGGAAGCCGTCAGA
		R: GCGAGGCCGTAAAGTGTAT
Ecdysone 20 monooxygenase like internal reference		F: GATTTAGCGGAAGCCGTCAG	59	36
		R: GCGAGGCCGTAAAGTGTAT
IMP-L2-like	253 (A/G)	F1: ACTTGCCAAGCCAAGTCTG	59.5	205
		F2: CACTTGCCAAGCCAAGTCTA
		R: TTCGAGCCACTTCCTTTTCG
IMP-L2-like internal reference		F: CTACACTTGCCAAGCCAAGTCT	59.5	207
		R: TTCGAGCCACTTCCTTTTCG

Notes.

The snp present is located at the ′ end of each forward primer (marked in red).

F1

Forward primer 1

F2

Forward primer 2

R

Common reverse primer

T_A

Annealing temperature