Table 3. Antibodies used for Western Blot Analysis.
| Antibody | Species | Dilution | Buffer | Incubation | Company (Cat.#) |
|---|---|---|---|---|---|
| MGAT1 | Mouse | 1:1,000 | Li-cor | 16 h 4°C | Proteintech (15103-1-AP) |
| MGAT4A | Mouse | 1:500 | 5% BSA | 16 h 4°C | Abnova (H00011320-M02) |
| MGAT4B | Mouse | 1:250 | 5% Milk | 16 h 4°C | Abnova (H00011282-M01) |
| MGAT4C | Rabbit | 1:250 | 5% Milk | 16 h 4°C | Sigma (HPA016418) |
| MGAT5 | Mouse | 1:1,000 | 5% Milk | 16 h 4°C | Abnova (H00004249-M09) |
| MGAT5B | Rabbit | 1:500 | Li-cor | 16 h 4°C | Proteintech (16993-1-AP) |
| B3GNT2 | Goat | 1:1,000 | Li-cor | 16 h 4°C | Abnova (PAB7522) |
| B3GNT3 | Mouse | 1:250 | 5% BSA | 16 h 4°C | Abnova (H00010331-A01) |
| B3GNT8 | |||||
| (B3GNTL1) | Rabbit | 1:2,000 | Li-cor | 16 h 4°C | Novus (NBP1-90838) |
| VCP | Mouse | 1:25,000 | Li-cor | 1 h RT | Abcam (AB11433) |
| VCP | Rabbit | 1:25,000 | Li-cor | 1 h RT | Abcam (AB36047) |
Abbreviations: Bovine serum albumin (BSA); Li-cor Odyssey blocking buffer (Li-cor)