Fig. 3.

Cytometric determination of intracellular-ROS quantified by DCFH-DA oxidation. Percentage of cells showing low (A) and high (B) intracellular ROS levels at study entry (pre) and following 2-week supplementation (post). Distribution of data relative to untreated control (ctrl), cells exposed to 100 µM (100) or 200 µM (200) H₂O₂ are reported. Data relative to 16 subjects are reported as box plot diagram where the cross (x), the box and the bars represent respectively the median, the 50% and 25% of data distribution. ***p<0.01 highly significant, **p<0.05 significant and *p<0.1 approaching significance compared to study entry.