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CTLA-4 Y60C is associated with impaired binding of its ligand CD80. CTLA-4 expression and CD80 binding as determined by flow cytometry in HEK293 cells after transfection of WT CTLA-4, Y60C CTLA-4 and empty plasmid (control). Total expression of CTLA-4 reflects combined surface and intracellular staining after cell permeabilisation.
