Fig. 4.

Projection of cell-size illumination patches through $40\times$ objective in conventional (non-TF) and TF modes. (a) Excitation patches matched to a selection of $10\mu \mathrm{m}$ fluorescent beads in order to cover their whole cross-section. (b) Images of different sized non-TF patches projected on thin fluorescein layer and averaged over five different holograms to reduce speckle noise. (c) Correspondence between illumination patch target diameter and their corresponding lateral dimensions (shown for non-TF mode). (d) Variable-sized illumination patches and their corresponding axial dimensions for both modes. Scale bars: $10\mu \mathrm{m}$.