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Thr855 MYPT phosphorylation was not significantly influenced by arachidonic acid, ROK inhibition or septic shock. The proportion of the ROK substrate MYPT phosphorylated at the inhibitory Thr855 site was not increased following stimulation with arachidonic acid and/or incubation with Y27632 and did not differ between samples from patients with septic shock (red bar) compared to samples from non-septic shock patients (blue bar) (n = 13 to 15 per patient group).
