. 2015 Jun 3;290(30):18281–18292. doi: 10.1074/jbc.M115.664664

TABLE 1.

Specific activities and kinetic parameters for the synthetic and phosphorolytic reactions of wild-type SdCBAP and its mutants

Enzyme	Synthesis acceptor								Phosphorolysis substrate CbA specific activity^a
	GlcA				GlcUA
	Specific activity^a	k_cat	K_m	k_cat/K_m	Specific activity^a	k_cat	K_m	k_cat/K_m
	units/mg	s⁻¹	mm	s⁻¹mm⁻¹	units/mg	s⁻¹	mm	s⁻¹mm⁻¹	units/mg
WT	93.2	130 ± 6	1.7 ± 0.2	76	10	34.6 ± 0.7	13.4 ± 0.5	2.58	62
Q190A	3.3	24 ± 3	41 ± 7	0.58	1.1	ND^b	ND	0.16	9.04
R609A	<0.005	ND	ND	ND	<0.001	ND	ND	ND	<0.001
K613A	<0.01	ND	ND	ND	<0.01	ND	ND	ND	<0.002

^a The specific activities were measured at 10 mm substrates.

^b ND, not determined.