FIGURE 5.

Localization of binding domains within C4BP and plasminogen. A and B, schematic representation of different C4BP α-chain deletion mutants (A) and various plasminogen derivatives (B) used. Black circles within each α-chain represent the C4BP α-chain deletion mutants lacking single CCP domains. Although for plasminogen, the kringle domains are depicted by K1 to K5 and the serine protease domain is depicted by P. C, the C4BP variants were allowed to bind to plasminogen immobilized on a plate. Bound C4BP was detected with polyclonal Abs. The graph represents data from three independent experiments done in duplicates ± S.D. Statistical significance was calculated using a one-way analysis of variance test. **, p = 0.01; ***, p = 0.001. D, microtiter plates were coated with variants of plasminogen (10 μg/ml) and incubated with plasma-purified C4BP-PS. Bound C4BP was detected using specific Abs. The data represent mean ± S.D. of three independent experiments performed in duplicate. Statistical significance was calculated using a two-way analysis of variance test. ns, not significant; *, p < 0.05; ***, p < 0.001.