FIGURE 1.

Overview of the hypusine modification and experimental design to study its role in mammals. A, schematic representation of hypusine synthesis in eIF-5A catalyzed by the DHS and DOHH enzymes. Red numbers in parentheses indicate the knock-out strategy for the respective gene as outlined in B. Hyp, hypusine; Dhp, deoxyhypusine. B, conditional knock-out strategy using two Cre-deleter mouse strains for either an early constitutive (1) and/or a 4-OHT-inducible (2) knock-out of the respective gene. Cre mouse strains are as follows: (1) = B6.C-Tg(CMV-cre)1Cgn/J; (2) = B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J113. C, time schedule for the 4-OHT-inducible knock-out (2) of the genes outlined in B.