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. 2015 Jun 2;290(30):18343–18360. doi: 10.1074/jbc.M115.664490

FIGURE 9.

FIGURE 9.

eIF-5A2 knock-out mice are viable and fertile. A, offspring analysis of the indicated breedings. B, Kaplan-Meier plot showing overall survival of mice at the indicated time points after birth. C, mRNA levels of eIF-5A2 in different tissues of wild type mice as assessed by quantitative real time PCR using the Tissue Scan qPCR Array MDRT101 (OriGene Technologies, Rockville, MD). Values were normalized to the expression of the housekeeping gene Gapdh and depicted as fold changes with bone marrow as the reference. D, mRNA status of eIF-5A2 in different organs of mice as assessed by quantitative real time PCR. Expression values were plotted relative to the expression of the housekeeping gene 18S. E, protein expression of eIF-5A2 in testis and brain of eIF-5A2−/− animals (n = 2) and eIF-5A2+/+ mice (n = 1) assessed by Western blot. eIF-5A1 expression served as a loading control. Organs were isolated from 7- to 10-month-old animals. F–H, mRNA status of eIF-5A1 as well as Dhs and Dohh in different organs of mice as assessed by quantitative real time PCR. Expression values were plotted relative to the expression of the housekeeping gene 18S. Significances were calculated using the unpaired t test and marked with an asterisk if significant (**, p < 0.001; *, p < 0.05). n.d., not detectable.