FIGURE 8.

THIP does not affect the spike frequency adaptation (SFA) in either WT or Mecp2-null neurons. A, the SFA was studied with series of depolarizing currents (0–0.15 nA). The firing rate of the neurons declined with a long period of depolarization. B, the SFA ratio was obtained by division of the peak frequency, measured between the first two APs, by the steady-state frequency, measured between the last two APs with the same current injection. The SFA ratio was increased with the increasing depolarizing currents. THIP treatment did not affect the SFA ratio in WT and Mecp2-null neurons. C, with a 0.06-nA current injection, THIP did not affected the SFA ratio in WT nor Mecp2-null neurons (n = 6 and 9; p > 0.05 and p > 0.05, respectively; Student's t test).