FIGURE 4.

A-Fabp does not compete with Atgl for Cgi-58 binding. In solid phase assays, polystyrene plates were coated with 1 μg of purified Atgl and incubated with bacterial lysates (60 μg of total protein) containing LacZ, A-Fabp, or mCgi-58 together with increasing amounts of purified Strep-A-Fabp (0.5-, 1-, or 5-fold molar excess compared with Atgl). Bound proteins were visualized by anti-Strep primary and Hrp-conjugated secondary antibody. Plates were developed using tetramethylbenzidine as substrate, and the absorbance was measured at 450/620 nm. Data are shown as mean ± S.D. (n = 4) and are representative of three independent experiments. Statistical difference was determined as compared with LacZ control (***, p < 0.001).