FIGURE 6.

A-Fabp promotes lipolysis solely in the presence of Cgi-58. a, TG hydrolase activities of cell lysates derived from COS-7 cells expressing His-tagged LacZ (control), Atgl, Cgi-58, and/or A-Fabp were determined by incubating lysates with phospholipid-emulsified triolein substrate containing radiolabeled triolein as tracer. After extraction of free FAs, radioactivity was determined by liquid scintillation counting. b, Western blot analysis of respective COS-7 lysates using anti-His primary and Hrp-conjugated secondary antibody. c, TG hydrolase activities of lysates prepared from differentiated 3T3-L1 adipocytes. Lysates were incubated with artificial substrate as in a in presence of increasing concentrations of the A-Fabp-inhibitor BMS309403 (Fi). d, TG hydrolase activities of lysates prepared from murine gonadal WAT. Lysates were incubated with artificial substrate as in a in presence of BMS309403 (Fi; 100 μm), the Atgl-inhibitor Atglistatin (Ai; 20 μm), and/or the Hsl inhibitor 76-0079 (Hi; 10 μm). Data are shown as mean ± S.D. (n = 3) and are representative of three independent experiments. Statistical difference was determined as compared with control (***, p < 0.001).