FIGURE 7.
L-Fabp promotes Pparα signaling. a, Western blot analysis of COS-7 cells that were transfected with a reporter plasmid coding for firefly luciferase under transcriptional control of multiple Ppar-responsive elements (PPRE), a plasmid coding for Renilla luciferase (transfection control), and a single plasmid or a mixture of plasmids coding for Pparα, Atgl, L-Fabp, Cgi-58, or the catalytically inactive AtglS47A (as indicated). b, for firefly luciferase gene transactivation assays, relative luminescence units of firefly and Renilla luciferase were determined in a 24-well plate luminometer and calculated relative to Renilla luciferase activities (control values were set to 100%). c, TG hydrolase activity (as in Fig. 6) assay and data are shown as mean ± S.D. (n = 4) and are representative of three independent experiments. Statistical difference was determined as compared with control (*, p < 0.05; **, p < 0.01; ***, p < 0.001).