FIGURE 8.
A-Fabp promotes Pparγ signaling. a and b, for firefly luciferase gene transactivation assays, COS-7 cells were transfected with the reporter plasmid coding for firefly luciferase under transcriptional control of multiple PPRE, a plasmid coding for Renilla luciferase (transfection control), and a plasmid or a mixture of plasmids coding for Pparγ, Atgl, A-Fabp, Cgi-58, the non-FA binding mutant variant A-FabpR127Q, or the mutant variant A-FabpF58A incapable of ligand-induced nuclear translocation (as indicated). Relative luminescence units of firefly and Renilla luciferase were determined in a 24-well plate luminometer and calculated relative to Renilla luciferase activities (control values were set to 100%). c, Western blot analysis of cytoplasmic and nuclear fractions obtained from differentiated 3T3-L1 adipocytes under nonstimulated (= basal, −F) and forskolin-stimulated (+F) conditions. A-Fabp was detected using an antibody specific for A-Fabp and Hrp-conjugated secondary antibody. Purity of the fractions was assessed by Western blotting using anti-histone H3 and anti-Gapdh antibodies, respectively. Data are shown as mean ± S.D. (n = 4) and are representative of three independent experiments. Statistical difference was determined as compared with control (*, p < 0.05; **, p < 0.01; ***, p < 0.001).