FIGURE 5.

Rkr1 regulates CPY-NS and CPY-K12 abundance. A and B, CPY, CPY-NS, and CPY-K12–13myc proteins were detected in yeast strains of the indicated genotypes by anti-ProtA Western blotting. The membrane in B was reprobed with an anti-Myc antibody. Pgk1 served as a loading control and was detected with an anti-Pgk1 antibody. C, lysates prepared from cells expressing the indicated constructs were incubated in the presence or absence of Endo H. See Fig. 2 for schematic depiction of constructs in this figure.