FIGURE 6.

Rkr1 regulates Vma12-K12 abundance. A and B, Vma12, Vma12-NS, and Vma12-K12–13myc proteins were detected in yeast strains of the indicated genotypes by anti-ProtA Western blotting. G6PDH served as a loading control and was detected with an anti-G6PDH antibody. C, N-glycosylation sequences were artificially inserted into the ER luminal loop of Vma12-K12–13myc to generate Vma12(glyc)-K12–13myc. Lysates from cells of the indicated genotypes expressing Vma12(glyc)-K12–13myc were incubated in the presence or absence of Endo H. The black lines indicate that intervening lanes have been spliced out. See Fig. 2 for schematic depiction of constructs in this figure.