FIGURE 7.

Rkr1 regulates OPY-K12 abundance. A, lysates prepared from cells expressing the indicated constructs were incubated in the presence or absence of Endo H. B and C, OPY, OPY-NS, and OPY-K12–13myc proteins were detected in yeast strains of the indicated genotypes by anti-ProtA Western blotting. Pgk1 served as a loading control and was detected with an anti-Pgk1 antibody. D, lysates from cells expressing CPY-K12–13myc, Vma12-K12–13myc, or OPY-K12–13myc were analyzed by anti-ProtA Western blotting. 20% volume of lysate harboring Vma12-K12–13myc was loaded to facilitate clearer comparison of relative abundance of truncated (K12) and full-length (K12–13myc) species. See Fig. 2 for schematic depiction of constructs in this figure.