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I-DmoI WT strand cleavage preference after phosphorothioate substitution. The figure displays time course titrations using phosphorothioate 6-fluorescein amidite (6FAM) labeling in coding strand A (Target 1) or coding strand B (Target 2). Oligonucleotides and I-DmoI at 750 nm were incubated at 65 °C from 0 to 60 min.
