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Amino acids 79–271 are required for interaction of LPCAT1 with StarD10. A, schematic of HA-tagged LPCAT1 mutants and full-length StarD10-FLAG constructs. B, co-IP of LPCAT1(1–271)-HA plus StarD10-FLAG showing that this fragment interacts with StarD10. C, co-IP analysis shows that mutant LPCAT1(79–271)-HA, a further truncation of LPCAT1, also interacts with StarD10-FLAG. NS, nonspecific band. Results are representative of three independent experiments.
