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. 2015 Jun 5;290(30):18559–18574. doi: 10.1074/jbc.M115.666701

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — The START domain of StarD10 is required for interaction with LPCAT1. A, schematic of HA- or FLAG-tagged full-length LPCAT1 and mutant StarD10 constructs. B and C, co-IP of LPCAT1 and StarD10 mutants. Co-IP of LPCAT1-FLAG plus StarD10(1–227)-HA resulted in pulldown of StarD10(1–227) by LPCAT1 (B), whereas co-IP of LPCAT1-HA plus StarD10(19–227)-FLAG resulted in pulldown of LPCAT1 by StarD10(19–227) (C), demonstrating that the START domain of StarD10 is sufficient for its interaction with LPCAT1. NS, nonspecific band. Results are representative of three independent experiments.