Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 5;290(30):18559–18574. doi: 10.1074/jbc.M115.666701

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 9. — LPCAT1 interacts with StarD7-I through its N terminus but does not interact with StarD2/PCTP. A, schematic of FLAG- or HA-tagged LPCAT1, StarD7, and StarD2/PCTP constructs. B, co-IP of LPCAT1-FLAG and StarD7-I-HA. Co-IP using LPCAT1-FLAG and StarD7-I-HA resulted in pulldown of StarD7-I by LPCAT1. The alternative splicing variant StarD7-II was detected but did not co-precipitate with LPCAT1. C, co-IP of LPCAT1-HA and StarD7-I(1–78)-FLAG. Co-IP with LPCAT1-HA and StarD7-I(1–78)-FLAG resulted in pulldown of LPCAT1 by StarD7-I(1–78), demonstrating that the N-terminal 78 amino acids of StarD7-I are sufficient for interaction with LPCAT1. NS, nonspecific band. D, StarD2/PCTP does not interact with LPCAT1. These data represent the results of five independent experiments.